Mechanism of DNA elongation catalyzed by Escherichia coli DNA polymerase III, dnaZ protein, and DNA elongation factors I and IIIS Wickner. Proc Natl Acad Sci U S A. 1976 Oct. Show
Free PMC article AbstractElongation of a primed single-stranded DNA template catalyzed by E. coli DNA polymerase III (DNA nucleotidyltransferase, deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) requires dnaZ protein and two other protein factors, DNA elongation factors I and III. The reaction occurs by the following mechanism: (i) dnaZ protein and DNA elongation factor III together catalyze the transfer of DNA elongation factor I to a primed DNA template. This transfer reaction requires ATP or dATP in addition to dnaZ protein, DNA elongation factors I and III, and primed template; it does not require DNA polymerase III. (ii) DNA polymerase III binds to the complex of DNA elongation factor I with primed template; it does not bind to primed template which is not complexed with DNA elongation factor I. This binding reaction proceeds in the absence of ATP or dATP as cofactor, dnaZ protein, and DNA elongation factor III and without additional DNA elongation factor I. (iii) The complex of DNA polymerase III, DNA elongation factor I, and primed template catalyzes DNA synthesis upon the addition of dNTPs. Similar articles
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Which enzyme catalyzes the elongation of the DNA strands in the 5 to 3 direction?DNA polymerase III holoenzyme (Pol III HE) is an enzyme that catalyzes elongation of DNA chains during bacterial chromosomal DNA replication.
Which enzyme catalyzes the elongation of a DNA strand in the 5 → 3 direction by adding nucleotides to the end of a growing DNA strand?DNA polymerase is the enzyme that catalyzes the addition of a nucleotide onto the 3' end of a growing DNA strand. DNA polymerase provides the free energy to catalyze the endergonic addition of a nucleotide onto the 3' end of a growing DNA strand. DNA polymerase catalyzes the synthesis of the template strand of DNA.
Which enzyme catalyzes the elongation of a DNA strand in the 5 → 3 direction quizlet?In E. coli, which enzyme catalyzes the elongation of a new DNA strand in the 5' → 3' direction? DNA polymerase can join new nucleotides only to the 3 end of a pre-existing strand, and the strands are antiparallel.
What synthesizes the new strand in a 5 to 3 direction?DNA polymerase only synthesizes DNA in the 5' to 3' direction only. The difference between the leading and lagging strands is that the leading strand is formed towards replication fork, while the lagging strand is formed away from replication fork.
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Which enzyme in E. coli catalyzes the elongation of a DNA strand in the 5 → 3 direction?
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