Patient should be instructed on the proper collection of a clean catch midstream urine specimen. Avoid contamination with normal flora from skin, rectum or vagina. If a clean catch urine cannot be obtained from an infant, obtain a bagged specimen: clean area as for a clean catch, attach U-bag, and put collected urine into a sterile container. Show Clean catch specimen: Have patient urinate into a small clean disposable cup (styrofoam or “Dixie”). Afterward, collection site staff can transfer the urine to the urine culture transport tube using the special collection straw-puncture device designed for use with the Vacutainer® tubes. The numbers of bacteria in a clean unused cup are so few as to be inconsequential when the urine transport stabilizer is added. Thoroughly instruct patient for proper collection of a “clean catch” specimen. Patient must be instructed to thoroughly cleanse skin and collect a midstream specimen. The patient should be instructed to follow the directions provided with the urine collection kit as follows. Catheterized specimen: Refers to an “in and out” catheter that is placed into the bladder solely for collection of the specimen and then withdrawn. Do not collect urine from the drainage bag when an indwelling catheter is in place because growth of bacteria can occur in the bag itself. Rather, clean catheter with an alcohol sponge, puncture with a sterile needle, and collect in sterile syringe. Catheter tips are contaminated by the urethra as they are withdrawn; do not culture them. Male: Wash hands thoroughly with soap and water. Rinse them well and dry with a paper towel. • Tear open the towelette packages so that the towels can be easily removed with one hand as they are needed. Do not touch any of the inside surfaces of the collection cup. • Pull back the foreskin to expose the head of the penis completely. • Wash the head of the penis thoroughly using first one towelette then the other. Discard the used towelettes into the toilet bowl. • Pass a small amount of urine into the toilet bowl, then pass a sample into the container. Do not allow the container to touch the legs or penis. Keep fingers away from the rim and inner surface of the container. Fill the container half full. • The urine specimen should be transferred to the Vacutainer® tube within 10 minutes of collection. Female: Wash hands thoroughly with soap and water. Rinse them well and dry with a paper towel. • Tear open the towelette packages so that the towels can be easily removed with one hand as they are needed. Do not touch any of the inside surfaces of the collection cup. • Remove undergarments and sit on the toilet seat with legs spread widely apart. • With one hand, spread labia apart to expose the vulva. Keep this hand in place during the washing and urinating procedure. • Use one towelette to wash the vulva well passing the towelette only from front to back, not back and forth. Repeat this procedure using the second towelette. Discard the used towelettes into the toilet bowl. • Begin urinating into the toilet bowl then, without stopping the stream, insert the collection cup to collect the specimen. Do not allow the container to touch the legs, vulva, or clothing. Keep fingers away from the rim and inner surface of the container. Fill the container about half full. • The urine specimen should be transferred to the Vacutainer® tube within 10 minutes of collection. Urine Collection Procedure URINE SPECIMENS I. PRINCIPLE Collection and transportation of urine specimens to the clinical laboratory are important because II. SPECIMEN REQUIREMENTS A. Specimen types 1. Random specimen 2. First morning specimen or 8-hour specimen 3. Fasting specimen 4. 2-Hour postprandial specimen 5. 24-hour (or timed) specimen 6. Catheterized specimen 7. Midstream “clean catch” specimen 8. Suprapubic aspiration 9. Pediatric specimens B. Transportation of specimens Urine specimens should be delivered to the within 2 hours of collection or refrigerated III. REAGENTS AND SUPPLIES (for collection of “clean catch” specimens) A. Disposable, clean, dry, leak-proof container (sterile container with lid required for B. Screw top specimen tube C. Disposable gloves D. Betadine swabs (Hibiclens if allergic to betadine) E. Dry, clean gauze F. Patient’s bedpan or urinal, if patient is unable to go the bathroom. IV. CALIBRATION No calibration is required for this procedure. V. QUALITY CONTROL Identification of the patient must be performed by asking a conscious patient his or her full name VI. PROCEDURE A. Patient preparation: For FEMALE patients: 1. Wash hands thoroughly before beginning the procedure and put on disposable 2. Use betadine swabs or Hibiclens to cleanse the perineal area. a. Separate the folds of the labia and wipe the betadine swab or Hibiclens from For MALE patients: 1. Wash hands thoroughly before beginning the procedure and put on disposable 2. If the patient is not circumcised, pull the foreskin back (retract the foreskin) on 3. Using a circular motion, clean the head of the penis with betadine swabs or B. Urination should begin, passing the first portion into the bedpan, urinal, or toilet. C. After the flow of urine has started, the urine specimen container should be placed under D. Any excess urine can pass into the bedpan, urinal, or toilet. E. Cover the urine container immediately with the lid being careful not to touch the inside of F. Transfer urine to specimen tube if tubes are used for transport instead of urine containers. G. Attach label to tube or container and place specimen in the transport bag. H. Remove gloves and wash hands. I. Record date and time of collection and initials of the person collecting (or submitting) the VII. CALCULATIONS Not applicable. VIII. REPORTING RESULTS Not applicable. IX. PROCEDURAL NOTES Specimens submitted for routine urinalysis should be collected in clean, dry containers. The The specimen containers must be properly labeled with appropriate patient identification A minimum of 12 ml of urine should be submitted for analysis. Smaller sample quantities will be SPECIMENS FOR PREGNANCY TESTING First morning specimens are the best for pregnancy testing because the urine is more concentrated. SPECIMENS FOR OSMOLALITY No special sample preparation is required. Whole blood, serum, plasma, or urine may be used. X. LIMITATIONS OF PROCEDURE A. Specimens submitted in syringes will not be accepted. What is the clinical application of routine urine culture?A urine culture is used to diagnose a urinary tract infection (UTI) and see what kinds of germs are causing it. The doctor may order a urine culture if your child: complains of a painful sensation when peeing. feels the urge to pee frequently but doesn't produce much urine (also called urgency)
What is the best specimen for urine culture?Early morning urine specimens are preferred, although urine collected at other times of the day are acceptable. A “mid-stream clean catch” urine sample is necessary for culture so that any bacteria present around the urethra and on the hands do not contaminate the specimen.
Which type of urine specimen collected in a sterile container is best for bacterial culture testing quizlet?The clean-catch, midstream specimen must be free of contamination because it is used to incubate bacteria. This is done to identify bacteria causing urinary infection.
Which urine specimen does not include first and last?What is a mid-stream urine sample? A mid-stream urine sample means you don't collect the first or last part of urine that comes out. This reduces the risk of the sample being contaminated with bacteria from: your hands.
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